Fwd: Need Help Processing with Image J

Posted by Emily Siska on
URL: http://imagej.273.s1.nabble.com/Need-Help-Processing-with-Image-J-tp5017774p5017775.html

---------- Forwarded message ----------
From: *Emily Siska* <[hidden email]>
Date: Tuesday, December 13, 2016
Subject: Need Help Processing with Image J
To: "Cammer, Michael" <[hidden email]>


Sorry for not attaching an example. Here are two examples. I also shared
them in dropbox.

On Tue, Dec 13, 2016 at 1:28 PM, Cammer, Michael <[hidden email]
<javascript:_e(%7B%7D,'cvml','[hidden email]');>> wrote:

> Post an example image to a fileserver and let people know so they can look
> at it.
>
> =========================================================================
>  Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical
> Center
>          Cell:  914-309-3270     Office: Skirball 2nd Floor main office,
> back right
>             http://ocs.med.nyu.edu/microscopy &
> http://microscopynotes.com/
>
>
> -----Original Message-----
> From: Emily Siska [mailto:[hidden email]
> <javascript:_e(%7B%7D,'cvml','[hidden email]');>]
> Sent: Tuesday, December 13, 2016 1:56 PM
> To: [hidden email]
> <javascript:_e(%7B%7D,'cvml','[hidden email]');>
> Subject: Need Help Processing with Image J
>
> Hello,
>
> I have SEM images of rubber fragments that I would like to process with
> Image J and I have been having a few problems. The rubber pieces do not
> have much contrast with the carbon tab that they are on and they are also
> not smooth - so there are lighter and darker spots of a paretic.  When
> using Image J I adjust the threshold until the particles and background
> have a high contrast. I try to count and find the perimeter and area of the
> parcels but the program seems to pick up random shapes on some particles
> instead of the whole thing. A friend said that its because although I see
> the particle as just white, the program is picking up differences and
> treating them like small individual particles. Is there any way around this?
>
>
> Thank you for all your help.
>
> ~Emily Siska
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--
Emily Siska

Chemistry Graduate Assistant
Chemistry/HiPSEC

Science and Engineering Building, Room 2173
University of Nevada, Las Vegas
4505 S. Maryland Pkwy.
Las Vegas, NV 89154-4003
Office: [phone]
Cell: [phone]



--
Emily Siska

Chemistry Graduate Assistant
Chemistry/HiPSEC

Science and Engineering Building, Room 2173
University of Nevada, Las Vegas
4505 S. Maryland Pkwy.
Las Vegas, NV 89154-4003
Office: [phone]
Cell: [phone]

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