Posted by
Krs5 on
Jan 25, 2017; 9:18am
URL: http://imagej.273.s1.nabble.com/thresholding-selecting-wrong-areas-tp5017937p5017942.html
Hi Joe,
Biovoxxel selects the setting Process > Binary > Options > Black background. You have to deselect this to restore the threshold function.
If you use Biovoxxel a lot you can add: run("Options...", "iterations=1 count=1"); to Edit > Options > Start Up to restores this setting when you restart ImageJ/Fiji
Best wishes
Kees
Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Uknalis, Joseph
Sent: 24 January 2017 16:20
To:
[hidden email]
Subject: thresholding selecting wrong areas
I was trying Adjustable Watershed and then Biovoxxel Watershed irregular features to separate large particles in an image. (w not much success) Now when I threshold the background gets selected and not the objects
Clicked/unclicked dark background in THRESHOLD, no luck Tried inverting, no luck Cleared imagej.cfg, no luck Upgraded version from 1.49q to 1.51i (via ij.jar replacement), no luck
Any ideas on how to restore this threshold function?
Thanks
Joe
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