Re: polygon plot profile

Posted by Michael P Ellis on Feb 21, 2017; 4:15pm
URL: http://imagej.273.s1.nabble.com/polygon-plot-profile-tp5018156p5018157.html

Just use the “Segmented Line Tool” in place of the Ploygon tool, then the Plot Profile will work.

“Segmented Line Tool” is available by meta-clicking on the Line Tool button in the tool bar.

You can also set the profile line thickness that gets used by double clicking on the “Line Tool” in the tool bar.

— Michael Ellis

> On 21 Feb 2017, at 17:54, Nirbio <[hidden email]> wrote:
>
> Hi There,
>
> I would appreciate if you could help me with the following issue: I am
> trying to get a plot profile for a polygonal shape. It seems like the
> software only accept rectangle or line shape for the analysis, resulting in
> averaging the signal over a range of pixels not occupied by my cells, hence
> reducing the "true signal". is there a way to get the plot profile for a
> polygon. This will be extremely helpful.
>
> Thanks in advance,
>
> Nir
>
>
>
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