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Automated Scratch Assay Analysis - doesn't work?

Posted by imagenoob on Feb 23, 2017; 12:26am
URL: http://imagej.273.s1.nabble.com/Automated-Scratch-Assay-Analysis-doesn-t-work-tp5018174.html

Hi guys,

I'm hoping to get imageJ to automatically measure the scratch performed on the scratch assay. I have downloaded the MRI Wound Healing Tool, but while it worked on sample images, if doesn't work on images created in our lab:




Does anyone have any tips? I've already tried playing around with the contrast, subtracting background, using Gaussian Blur, to no effect.

Many thanks for your time!
CL