Automated Scratch Assay Analysis - doesn't work?
Posted by
imagenoob on
Feb 23, 2017; 12:26am
URL: http://imagej.273.s1.nabble.com/Automated-Scratch-Assay-Analysis-doesn-t-work-tp5018174.html
Hi guys,
I'm hoping to get imageJ to automatically measure the scratch performed on the scratch assay. I have downloaded the MRI Wound Healing Tool, but while it worked on sample images, if doesn't work on images created in our lab:


Does anyone have any tips? I've already tried playing around with the contrast, subtracting background, using Gaussian Blur, to no effect.
Many thanks for your time!
CL