Normalizing GCaMP signal with mCherry signal

Posted by smilingtoad on
URL: http://imagej.273.s1.nabble.com/Normalizing-GCaMP-signal-with-mCherry-signal-tp5018221.html

Greetings image enthusiasts -

Alex here, I am PhD graduate student with a quick image normalization question.  I have been doing some imaging of the organ of asymmetry in zebrafish (Kupffer's Vesicle) using GCaMP to detect calcium elevations around this organ and using mCherry expressed 1:1 in order to control for cell to cell expression variation.

My question is what is the best method to "normalize" my GCaMP signal using mCherry signal.  I initially though something like the Image Process: Divide function would give me what I need but now reading around the net I am not so sure.   Any and all advice is welcome and appreciated   thanks!!