Login  Register

ROI to calculate mean grey value of 2 stacks

Posted by Nan on Mar 23, 2017; 5:48am
URL: http://imagej.273.s1.nabble.com/ROI-to-calculate-mean-grey-value-of-2-stacks-tp5018354.html

I'm a fresh new in ImageJ, and the problem comes from calcutating fluorescence intensity of 2 different stacks.
For only one stack, it always goes easier by following steps :Import image sequence --> Set Measurement --> Select a 'ROI' --> Press 't' to add in ROI manager --> More --> Multi Measure the fluorescence intensity of all slices is done with the result showing in Result window. However, I would like to measure the same value in two different stacks at the same time, the selected ROIs are messed up and only one of them can be processed with measurement. Is there a solution for it?

Best regards,
Nan