Posted by
Swayne, Theresa C. on
Mar 23, 2017; 5:40pm
URL: http://imagej.273.s1.nabble.com/ROI-to-calculate-mean-grey-value-of-2-stacks-tp5018354p5018360.html
Hi Nan,
However, I would like to measure the same value in two different stacks at the same time
Measurement is done on one image window at a time. You can’t measure 2 different stacks “at the same time.” But you can write a macro to measure each in turn.
As an alternative, when you do ROI Manager > More > Multi-Measure, check the box “Append Results.”
Then clear the Results table, measure the first stack, then measure the 2nd stack.
The results from the 2nd stack will be added to the Results table right after the results from the 1st stack.
the selected ROIs are messed up
Could you give more details on this? What steps do you take, what is the result, and how is the result different from what you want?
Ideally, you could attach or link to images that illustrate the issue.
Hope this helps.
On Mar 23, 2017, at 1:49 AM, Nan <
[hidden email]<mailto:
[hidden email]>> wrote:
I'm a fresh new in ImageJ, and the problem comes from calcutating fluorescence intensity of 2 different stacks.
For only one stack, it always goes easier by following steps :Import image sequence --> Set Measurement --> Select a 'ROI' --> Press 't' to add in ROI manager --> More --> Multi Measure the fluorescence intensity of all slices is done with the result showing in Result window. However, I would like to measure the same value in two different stacks at the same time, the selected ROIs are messed up and only one of them can be processed with measurement. Is there a solution for it?
Best regards,
Nan
--
View this message in context:
http://imagej.1557.x6.nabble.com/ROI-to-calculate-mean-grey-value-of-2-stacks-tp5018354.htmlSent from the ImageJ mailing list archive at Nabble.com<
http://Nabble.com>.
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html------------------------------------
Theresa Swayne, Ph.D.
Manager
Confocal and Specialized Microscopy Shared Resource<
http://hiccc.columbia.edu/research/sharedresources/confocal>
Herbert Irving Comprehensive Cancer Center
Columbia University Medical Center
1130 St. Nicholas Ave., Room 222A
New York, NY 10032
Phone: 212-851-4613
[hidden email]<mailto:
[hidden email]>
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html