Re: Quantification of axon Twist

Posted by Herbie on Mar 23, 2017; 6:59pm
URL: http://imagej.273.s1.nabble.com/Quantification-of-axon-Twist-tp5018355p5018361.html

Dear Eric,

thanks for your explanation concerning the source of your image. So the
neurons are grown on glass...

re: Orientation Salience Function

Here are links for two papers dealing with theoretical and algorithmic
aspects. If you don't have much time, please try to understand at least
the first two pages of the report (2013).

<http://www.gluender.de/Writings/WritingsTexts/WritingsDownloads/1986_IntegralsCentralSlices.zip>
<http://www.gluender.de/Writings/WritingsTexts/WritingsDownloads/2013_Orientation.zip>

In fact Orientation Salience Functions are weighted histograms and could
be normalized as such. Weighted, because they don' reflect the pure
geometric property of orientation but the contrast/brightness of the
image structures as well.

With your image, it is interesting that the 90deg bending of the axons
shows up nicely by the decline of the function for angles greater 90deg.
The bending itself is reflected by the plateau between 20deg and 90deg.
Beyond 90deg the orientations are less regular.

The generation of the Orientation Salience Function is done with ImageJ
by use of a dedicated plugin (cf. the 2013 report) and a macro.

I shall write you off-list tomorrow for more details.

Best

Herbie

:::::::::::::::::::::::::::::::::::::::::::
Am 23.03.17 um 12:58 schrieb Eric Denarier:

> Hi Herbie,
>
> Thanks for you help. Image is  actually Dorsal Root Ganglia neurons
> grown on coverslip (part of the image).
>
> How did you get the the orientation Salience ? With imageJ ?
>
> Eric Denarier
> Grenoble Institut des Neurosciences
> Inserm U1216
> Chemin Fortuné Ferrini
> 38700 La Tronche
> France
>
> Tél :[phone]
>
> http://neurosciences.ujf-grenoble.fr/
>
> Le 23/03/2017 à 10:12, Herbie a écrit :
>> orientation salience function
>
>

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