Posted by
Thomas Eschner on
Mar 28, 2017; 9:19am
URL: http://imagej.273.s1.nabble.com/Calculator-Plus-tp5018377p5018402.html
Hi Curtis, hi all,
thank you for your input. As i understand - the quirk is there, but there's also a workaround. That should do it at the moment.
Thanks once more.
Kind regards,
Thomas
Gesendet: Montag, 27. März 2017 um 18:03 Uhr
Von: "Curtis Rueden" <
[hidden email]>
An:
[hidden email]
Betreff: Re: Calculator Plus
Hi Thomas,
I usually do these sorts of comparisons using the Image Calculator command,
subtracting one image from the other, and checking the "32-bit result" box.
This is fine if your goal is to illustrate visually as you say. For
quantitative comparison, I agree with Joel that it's better to be more
rigorous, with something like colocalization.
Regards,
Curtis
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Curtis Rueden
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On Mon, Mar 27, 2017 at 6:50 AM, Thomas Eschner <
[hidden email]>
wrote:
> Dear all,
>
> I am trying to illustrate artefacts introduced by storing files as jpg by
> showing the difference of two identical 16-bit images, one stored as jpg
> and one as tiff.
>
> With Calculator Plus, setting e.g. Subtract with k1 = 1 and k2 = 16, I end
> up with files having their values set to 65535 exclusively.
>
> Only with k2 = 0 I seem to get more or less reasonable results, but I lose
> negative values.
>
> Could please anybody give me a hint?
>
> Thank you.
>
> Kind regards,
> Thomas
>
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