Re: Grid Stitching - no output image

Posted by Rossi, Sharyn (NIH/NIA/IRP) [C] on
URL: http://imagej.273.s1.nabble.com/Grid-Stitching-no-output-image-tp5018380p5018456.html

Hi Stephan,

Thanks for your reply. Yes – there are many images that were obtained using a light-sheet ultramicroscope so there is that in addition to the size of each individual image. I did select down sampling and cut the pixel size in half and I did specify the 20% overlap (although I also tried it without and obtained the same result). Do you think I should down-sample even more? I have 2 filters in the dataset so if you think it would help, I could separate the filters – stitch one color at a time and then overlay.

Thanks for your help,

Sharyn

On 4/6/17, 1:28 AM, "[hidden email]" <[hidden email]> wrote:

    Hi, your images are too big for the current version (?>2^31 pixels) … which parameters do you use to run the Stitching? It seems to me you might not specify an approximate overlap between tiles?
   
    Cheers,
    Stephan
    ---
   
    Dr. Stephan Preibisch
    Group Leader
   
    Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC)
    Building 89, 1.08b
   
    email: [hidden email]<mailto:[hidden email]>
    web: http://preibischlab.mdc-berlin.de
   
    On 5 Apr 2017, at 21:26, Rossi, Sharyn (NIH/NIA/IRP) [C] <[hidden email]<mailto:[hidden email]>> wrote:
   
    Hi,
   
    Figured I would try this once again to see if anyone else has encountered a problem like this. I am attaching the log and console info.
   
    Thanks,
   
    Sharyn
   
    On 3/27/17, 10:43 AM, "Rossi, Sharyn (NIH/NIA/IRP) [C]" <[hidden email]<mailto:[hidden email]>> wrote:
   
       Hello,
   
       I have run a large dataset obtained from an ultra-microscope through the grid-stitch plugin and although it generates an output.txt file and processes each of the images in the mosaic (as seen via console), I get no output image. I have tried writing to disk as well as the live stitch option to no avail. Any comments or suggestions as to what may be the problem?
   
       Thanks,
   
       Sharyn
   
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    <console.txt><Log.txt><Exception.txt>
   
   
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