16 bit image visualization

Posted by Knecht, David on Apr 27, 2017; 9:14pm
URL: http://imagej.273.s1.nabble.com/16-bit-image-visualization-tp5018625.html

When we collect 16 bit images, it is sometimes because we want the dynamic range to visualize structures or cells that are of wide ranging intensities.  However, as far as I know, it is difficult to isolate different intensity ranges in a 16 bit image and then play with the brightness/contrast of those pixels.  What I think I am looking for is a tool like the Adjust Contrast/Brightness sliders, but be able to set values above maximum the slider value to 0.  Or a tool like the Adjust Threshold tool, but instead of highlighting the pixels it would set pixels outside the selected range to black.  Another way of looking at this would be the equivalent of the min and max transparency bound in a projection.  I am wondering if such a tool is available in ImageJ or somewhere in the plugin universe and I have just not found it.  Thanks- Dave

Dr. David Knecht
Professor , Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd.
U-3125
Storrs, CT 06269-3125
860-486-2200


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