Posted by
Jeremy Adler-2 on
Apr 28, 2017; 9:09am
URL: http://imagej.273.s1.nabble.com/16-bit-image-visualization-tp5018625p5018630.html
clarification of earlier post
Adjust brightness and contrast works with the LUT used by your image.
You need to save your new lut (max set to zero) in the LUTs folder of ImageJ and when you restart it will appear in the list of LUTs.
So you need to display your image in the new LUT before running brightness & constrast.
________________________________________
From: ImageJ Interest Group [
[hidden email]] on behalf of Jeremy Adler [
[hidden email]]
Sent: 28 April 2017 05:51
To:
[hidden email]
Subject: Re: 16 bit image visualization
It is very easy to set the display of intensities above the selected threshold to zero - or any other value.
Use Image/ Color/ Edit LUT
and set the display values for the maximum intensity to 0,
then save the LUT.
________________________________________
From: ImageJ Interest Group [
[hidden email]] on behalf of Knecht, David [
[hidden email]]
Sent: 27 April 2017 23:14
To:
[hidden email]
Subject: 16 bit image visualization
When we collect 16 bit images, it is sometimes because we want the dynamic range to visualize structures or cells that are of wide ranging intensities. However, as far as I know, it is difficult to isolate different intensity ranges in a 16 bit image and then play with the brightness/contrast of those pixels. What I think I am looking for is a tool like the Adjust Contrast/Brightness sliders, but be able to set values above maximum the slider value to 0. Or a tool like the Adjust Threshold tool, but instead of highlighting the pixels it would set pixels outside the selected range to black. Another way of looking at this would be the equivalent of the min and max transparency bound in a projection. I am wondering if such a tool is available in ImageJ or somewhere in the plugin universe and I have just not found it. Thanks- Dave
Dr. David Knecht
Professor , Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd.
U-3125
Storrs, CT 06269-3125
860-486-2200
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