Re: Deconvolution Lab "Batch" Module

Posted by Fairy on
URL: http://imagej.273.s1.nabble.com/Deconvolution-Lab-Batch-Module-tp5015776p5018779.html

hii

thank you for replying me..yes..i do have multiple bead...the psf has 99 frames and image has 37 frames....if i am cropping a single bead out then how should i adjust the size to make it compatible with the raw image???because raw images size is 2048*2048 and bead size is 512......if i crop out a single bead, then the bead file will become more smaller!!!

On Tue, May 23, 2017 at 8:00 PM, bnorthan [via ImageJ] <[hidden email]> wrote:

Hi Ash

If you can share your image and psf I can try and help you.  From your
description I suspect your image contains multiple beads.  To use it as a
PSF you would have to crop out one bead.

Brian

On Mon, May 15, 2017 at 5:45 PM, Ash <[hidden email]> wrote:

> my input image size is 2048*2048*37 and psf size is 512*512*99....how
> should
> i resize it?do i have to do fft or ifft to get deconvolution result..the
> psf
> file that i have contain several beads....should i take average of all the
> beads in that file to deconvolution.... or just uploading the psf file with
> sveral beads is fine?
> i am doing with deconvolution lab software...how should i proceed?
>
>
>
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