Re: Deconvolution Lab "Batch" Module

Posted by bnorthan on
URL: http://imagej.273.s1.nabble.com/Deconvolution-Lab-Batch-Module-tp5015776p5018792.html

Hi Ash

If you are using deconvolution lab, it should automatically resize the PSF
in x and y.  If I recall correctly deconvolution lab does not resize in z.
So you should make the PSF x by y by 37 (z same z in image).  Make x and y
the right size to contain one bead.  Make sure the bead is centered.

I also would subtract the background level (at least subtract the min value
of the cropped bead image).

If you are able to share any images I can give it a try.

On Wed, May 24, 2017 at 4:54 AM, Ash <[hidden email]> wrote:

> hii
> thank you for replying me..yes..i do have multiple bead...the psf has 99
> frames and image has 37 frames....if i am cropping a single bead out then
> how should i adjust the size to make it compatible with the raw
> image???because raw images size is 2048*2048 and bead size is 512......if i
> crop out a single bead, then the bead file will become more smaller!!!
>
> On Tue, May 23, 2017 at 8:00 PM, bnorthan [via ImageJ] <
> [hidden email]> wrote:
>
> > Hi Ash
> >
> > If you can share your image and psf I can try and help you.  From your
> > description I suspect your image contains multiple beads.  To use it as a
> > PSF you would have to crop out one bead.
> >
> > Brian
> >
> > On Mon, May 15, 2017 at 5:45 PM, Ash <[hidden email]
> > <http:///user/SendEmail.jtp?type=node&node=5018778&i=0>> wrote:
> >
> > > my input image size is 2048*2048*37 and psf size is 512*512*99....how
> > > should
> > > i resize it?do i have to do fft or ifft to get deconvolution
> result..the
> > > psf
> > > file that i have contain several beads....should i take average of all
> > the
> > > beads in that file to deconvolution.... or just uploading the psf file
> > with
> > > sveral beads is fine?
> > > i am doing with deconvolution lab software...how should i proceed?
> > >
> > >
> > >
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