Background substracting except for a ROI

Posted by Adrián Villalba on Jun 07, 2017; 8:34am
URL: http://imagej.273.s1.nabble.com/Background-substracting-except-for-a-ROI-tp5018852.html

Dear all,

I use to substract background in fluorescence images (1 channel) by
substracting the mean value setted by measurements. In the case that the
background is not uniform i would like to know if it possible to substract
the entire image without the ROI from the original image.

 Thank you all in advance,

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   - Adrián Villalba Felipe.
   https://es.linkedin.com/in/adrianvillalba

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