Re: Background substracting except for a ROI

Posted by Krs5 on Jun 07, 2017; 3:06pm
URL: http://imagej.273.s1.nabble.com/Background-substracting-except-for-a-ROI-tp5018852p5018857.html

Dear Adrian,

You can use Process > Image Calculator to subtract images. There is also the option to use Process > Subtract Background... what uses a rolling background approach to correct uneven illuminated background, see: https://imagej.nih.gov/ij/docs/guide/146-29.html#toc-Subsection-29.14

Kees

Dr Ir K.R. Straatman
Senior Experimental Officer
Advanced Imaging Facility
Centre for Core Biotechnology Services
University of Leicester
http://www2.le.ac.uk/colleges/medbiopsych/facilities-and-services/cbs/lite/aif


-----Original Message-----
From: Adrián Villalba [mailto:[hidden email]]
Sent: 07 June 2017 09:35
To: [hidden email]
Subject: Background substracting except for a ROI

Dear all,

I use to substract background in fluorescence images (1 channel) by substracting the mean value setted by measurements. In the case that the background is not uniform i would like to know if it possible to substract the entire image without the ROI from the original image.

 Thank you all in advance,

--

   - Adrián Villalba Felipe.
   https://es.linkedin.com/in/adrianvillalba

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html