How to set the background value to the same value for a stack of images

Posted by jklwp007 on
URL: http://imagej.273.s1.nabble.com/How-to-set-the-background-value-to-the-same-value-for-a-stack-of-images-tp5018903.html

Hello everyone,

I am new here, and I have a question about set up background value of a stack of images. I got a lot time-lapse images (70 images for a stack). Due to the fluctuation of the illumination, I can not get a stable trace out of the stack. I want to set the background to a same value of all images of one stack. I can not find any macro or plugins that is helpful. Does anyone know how to solve this problem? I think the stupidest way is to subtract each single image to a same value of background, but it is too waste of time. I have too much images to deal with. I appreciate anyone that can help me out.

Thank you!