http://imagej.273.s1.nabble.com/How-to-set-the-background-value-to-the-same-value-for-a-stack-of-images-tp5018903p5018913.html
Dne 16. 6. 2017 7:43 odp. napsal uživatel "Michael Schmid" <
> Hi anonymous,
>
> Michael's macro assumes that area and brightness of the foreground objects
> are roughly the same for all stack slices and there is mostly background in
> the image.
>
> If this is not the case, for 8-bit images you could try using the Modal
> Gray Value instead of the Mean. Omit the "n*getResult("StdDev",(i-1)".
> Unfortunately, for 16-bit images the Modal Gray Value gives unreliable
> results because of binning the pixel values to 256 bins; some bins contain
> more 16-bit values than others. In that case, you have to retrieve the
> 16-bit histogram and process it yourself.
>
> You could also try using the Minimum or Maximum gray value (depends on
> whether you have a dark or bright background) instead of the Mean, but this
> is very sensitive to noise and outliers. A better alternative would be
> creating a duplicate of the stack, smoothing it or filtering by a median
> filter, and using the min or max gray value of this filtered stack.
>
> Please also note that Michael's macro is for images without pixel value
> calibration, and there should be no active selection (ROI) when it is run -
> you might add 'run("Select None");' to ensure this is the case.
>
> - Another Michael
> ________________________________________________________________
> On 16/06/2017 19:16, jklwp007 wrote:
>
>> Hi,
>>
>> Thank you for your quick replying. I tried this on a couple of my images,
>> but it did not uniform the background. Should I change any parameter in
>> that
>> macro?
>>
>>
>> --
>> View this message in context:
http://imagej.1557.x6.nabble.c>> om/How-to-set-the-background-value-to-the-same-value-for-a-s
>> tack-of-images-tp5018903p5018910.html
>>
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>