Re: Deconvolution Lab "Batch" Module

Posted by Fairy on
URL: http://imagej.273.s1.nabble.com/Deconvolution-Lab-Batch-Module-tp5015776p5018963.html

so before going to deconvolutionLab plugin, first do we have to convolve the image and its PSF?? And then compare results of image convolved with PSF , with the end result of that blurred image with PSF???

Or are we directly starting to deconvolve image that we have taken and its PSF???

On Sat, May 27, 2017 at 5:19 PM, bnorthan [via ImageJ] <[hidden email]> wrote:

Hi Ash

If you are using deconvolution lab, it should automatically resize the PSF
in x and y.  If I recall correctly deconvolution lab does not resize in z.
So you should make the PSF x by y by 37 (z same z in image).  Make x and y
the right size to contain one bead.  Make sure the bead is centered.

I also would subtract the background level (at least subtract the min value
of the cropped bead image).

If you are able to share any images I can give it a try.

On Wed, May 24, 2017 at 4:54 AM, Ash <[hidden email]> wrote:

> hii
> thank you for replying me..yes..i do have multiple bead...the psf has 99
> frames and image has 37 frames....if i am cropping a single bead out then
> how should i adjust the size to make it compatible with the raw
> image???because raw images size is 2048*2048 and bead size is 512......if i
> crop out a single bead, then the bead file will become more smaller!!!
>
> On Tue, May 23, 2017 at 8:00 PM, bnorthan [via ImageJ] <
> [hidden email]> wrote:

>
> > Hi Ash
> >
> > If you can share your image and psf I can try and help you.  From your
> > description I suspect your image contains multiple beads.  To use it as a
> > PSF you would have to crop out one bead.
> >
> > Brian
> >
> > On Mon, May 15, 2017 at 5:45 PM, Ash <[hidden email]

> > <http:///user/SendEmail.jtp?type=node&node=5018778&i=0>> wrote:

> >
> > > my input image size is 2048*2048*37 and psf size is 512*512*99....how
> > > should
> > > i resize it?do i have to do fft or ifft to get deconvolution
> result..the
> > > psf
> > > file that i have contain several beads....should i take average of all
> > the
> > > beads in that file to deconvolution.... or just uploading the psf file
> > with
> > > sveral beads is fine?
> > > i am doing with deconvolution lab software...how should i proceed?
> > >
> > >
> > >
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