http://imagej.273.s1.nabble.com/Deconvolution-Lab-Batch-Module-tp5015776p5018964.html
> so before going to deconvolutionLab plugin, first do we have to convolve
> the image and its PSF?? And then compare results of image convolved with
> PSF , with the end result of that blurred image with PSF???
>
> Or are we directly starting to deconvolve image that we have taken and its
> PSF???
>
> On Sat, May 27, 2017 at 5:19 PM, bnorthan [via ImageJ] <
>
[hidden email]> wrote:
>
> > Hi Ash
> >
> > If you are using deconvolution lab, it should automatically resize the
> PSF
> > in x and y. If I recall correctly deconvolution lab does not resize in
> z.
> > So you should make the PSF x by y by 37 (z same z in image). Make x and
> y
> > the right size to contain one bead. Make sure the bead is centered.
> >
> > I also would subtract the background level (at least subtract the min
> > value
> > of the cropped bead image).
> >
> > If you are able to share any images I can give it a try.
> >
> > On Wed, May 24, 2017 at 4:54 AM, Ash <[hidden email]
> > <
http:///user/SendEmail.jtp?type=node&node=5018792&i=0>> wrote:
> >
> > > hii
> > > thank you for replying me..yes..i do have multiple bead...the psf has
> 99
> > > frames and image has 37 frames....if i am cropping a single bead out
> > then
> > > how should i adjust the size to make it compatible with the raw
> > > image???because raw images size is 2048*2048 and bead size is
> > 512......if i
> > > crop out a single bead, then the bead file will become more smaller!!!
> > >
> > > On Tue, May 23, 2017 at 8:00 PM, bnorthan [via ImageJ] <
> > > [hidden email] <
http:///user/SendEmail.jtp?
> type=node&node=5018792&i=1>>
> > wrote:
> > >
> > > > Hi Ash
> > > >
> > > > If you can share your image and psf I can try and help you. From
> your
> > > > description I suspect your image contains multiple beads. To use it
> > as a
> > > > PSF you would have to crop out one bead.
> > > >
> > > > Brian
> > > >
> > > > On Mon, May 15, 2017 at 5:45 PM, Ash <[hidden email]
> > > > <
http:///user/SendEmail.jtp?type=node&node=5018778&i=0>> wrote:
> > > >
> > > > > my input image size is 2048*2048*37 and psf size is
> > 512*512*99....how
> > > > > should
> > > > > i resize it?do i have to do fft or ifft to get deconvolution
> > > result..the
> > > > > psf
> > > > > file that i have contain several beads....should i take average of
> > all
> > > > the
> > > > > beads in that file to deconvolution.... or just uploading the psf
> > file
> > > > with
> > > > > sveral beads is fine?
> > > > > i am doing with deconvolution lab software...how should i proceed?
> > > > >
> > > > >
> > > > >
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