Hi Ash
The dynamic range of a deconvolved image will be larger. So the output
probably will not scale to 8 bit.
Also make sure you click the option 'normalize PSF'. This will make the
PSF is normalized to unit energy, and that will mean you can directly
compare input and output values.
The deconvolved image should have sharper and higher peaks. This is
because blurry "wide" features, should now be narrower and more intense.
Brian
On Wed, Jun 28, 2017 at 7:06 AM, Ash <[hidden email]> wrote:
> i got results..thanks..one more doubt!!
> my input image was 8 bits..after deconvolution size is becoming 32
> bits...how do i convert output images intensity to that of input image??
>
> i want to compare the images...but since intensities are varying i m not
> able to compare!!
>
> On Sun, Jun 25, 2017 at 11:46 PM, Ash Fairy <[hidden email]> wrote:> >> <http:///user/SendEmail.jtp?
>
> > Thank you, I will do that. ..
> > Well can I send it to your mail id?
> >
> > On Jun 25, 2017 6:16 PM, "bnorthan [via ImageJ]" <ml+s1557n5018964h66@n6.
> > nabble.com> wrote:
> >
> >> Hi Ash
> >>
> >> If I understand you correctly, you need to directly deconvolve the
> image.
> >> The microscope has already approximately convolved the true object with
> >> the
> >> PSF.
> >>
> >> If you are able to share the image and bead image, I am happy to try and
> >> deconvolve them, and let you know the best approach and settings.
> >>
> >> Brian
> >>
> >> On Sun, Jun 25, 2017 at 12:09 AM, Ash <[hidden email]type=node&node=5018964&i=0 >> wrote:> >> > [hidden email] <http:///user/SendEmail.jtp?
> >>
> >> > so before going to deconvolutionLab plugin, first do we have to
> >> convolve
> >> > the image and its PSF?? And then compare results of image convolved
> >> with
> >> > PSF , with the end result of that blurred image with PSF???
> >> >
> >> > Or are we directly starting to deconvolve image that we have taken and
> >> its
> >> > PSF???
> >> >
> >> > On Sat, May 27, 2017 at 5:19 PM, bnorthan [via ImageJ] <
> type=node&node=5018964&i=1>>> >> > com/Deconvolution-Lab-Batch-> >> wrote:
> >> >
> >> > > Hi Ash
> >> > >
> >> > > If you are using deconvolution lab, it should automatically resize
> >> the
> >> > PSF
> >> > > in x and y. If I recall correctly deconvolution lab does not resize
> >> in
> >> > z.
> >> > > So you should make the PSF x by y by 37 (z same z in image). Make x
> >> and
> >> > y
> >> > > the right size to contain one bead. Make sure the bead is centered.
> >> > >
> >> > > I also would subtract the background level (at least subtract the
> min
> >> > > value
> >> > > of the cropped bead image).
> >> > >
> >> > > If you are able to share any images I can give it a try.
> >> > >
> >> > > On Wed, May 24, 2017 at 4:54 AM, Ash <[hidden email]
> >> > > <http:///user/SendEmail.jtp?type=node&node=5018792&i=0 >> wrote:
> >> > >
> >> > > > hii
> >> > > > thank you for replying me..yes..i do have multiple bead...the psf
> >> has
> >> > 99
> >> > > > frames and image has 37 frames....if i am cropping a single bead
> >> out
> >> > > then
> >> > > > how should i adjust the size to make it compatible with the raw
> >> > > > image???because raw images size is 2048*2048 and bead size is
> >> > > 512......if i
> >> > > > crop out a single bead, then the bead file will become more
> >> smaller!!!
> >> > > >
> >> > > > On Tue, May 23, 2017 at 8:00 PM, bnorthan [via ImageJ] <
> >> > > > [hidden email] <http:///user/SendEmail.jtp?
> >> > type=node&node=5018792&i=1>>
> >> > > wrote:
> >> > > >
> >> > > > > Hi Ash
> >> > > > >
> >> > > > > If you can share your image and psf I can try and help you.
> From
> >> > your
> >> > > > > description I suspect your image contains multiple beads. To
> use
> >> it
> >> > > as a
> >> > > > > PSF you would have to crop out one bead.
> >> > > > >
> >> > > > > Brian
> >> > > > >
> >> > > > > On Mon, May 15, 2017 at 5:45 PM, Ash <[hidden email]
> >> > > > > <http:///user/SendEmail.jtp?type=node&node=5018778&i=0 >> wrote:
> >> > > > >
> >> > > > > > my input image size is 2048*2048*37 and psf size is
> >> > > 512*512*99....how
> >> > > > > > should
> >> > > > > > i resize it?do i have to do fft or ifft to get deconvolution
> >> > > > result..the
> >> > > > > > psf
> >> > > > > > file that i have contain several beads....should i take
> average
> >> of
> >> > > all
> >> > > > > the
> >> > > > > > beads in that file to deconvolution.... or just uploading the
> >> psf
> >> > > file
> >> > > > > with
> >> > > > > > sveral beads is fine?
> >> > > > > > i am doing with deconvolution lab software...how should i
> >> proceed?
> >> > > > > >
> >> > > > > >
> >> > > > > >
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