Re: Segmenting z-stacks for 3D model
Posted by
Василий Попков on
Jul 03, 2017; 12:57pm
URL: http://imagej.273.s1.nabble.com/Segmenting-z-stacks-for-3D-model-tp5019024p5019026.html
Hi.
You might want to check this article and what (and how) they have done.
http://www.sciencedirect.com/science/article/pii/S2405471217300522Though, as I know, there is still no easy and established way to do what
you want.
On Mon, Jul 3, 2017 at 1:25 PM, Fly Guy <
[hidden email]> wrote:
> Hello ImageJers’
>
> I have a set of z-stacks of adult Drosophila brains where some of the
> cells (neural stem cells and neurons) are GFP-positive.
> There is also a bit of GFP+ background.
> I want to segment and reconstruct a 3D model of the GFP+ cells only
> (obviously without the background). Then I want to count the cells and
> measure their volume.
> After fiddling around with binary masks, 3D ROI and 3D segmentation, I am
> still at a loss for a ‘standardised’ way.
>
> Can someone kindly suggest a plugin etc or a methodology to solve the
> problem?
>
> Thanks and Best
> FlyGuy
>
>
>
>
>
>
> ***************************
> With thanks and Best Wishes
> Fly Guy
> Twitter: @flyguy
>
>
>
>
>
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>
--
Vasily A Popkov
Lab.Structure and function of mitochondria
A.N.Belozersky Institute of Physico-Chemical Biology
Moscow State University
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html