> Hello ImageJers’
>
> I have a set of z-stacks of adult Drosophila brains where some of the cells (neural stem cells and neurons) are GFP-positive.
> There is also a bit of GFP+ background.
> I want to segment and reconstruct a 3D model of the GFP+ cells only (obviously without the background). Then I want to count the cells and measure their volume.
> After fiddling around with binary masks, 3D ROI and 3D segmentation, I am still at a loss for a ‘standardised’ way.
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> Can someone kindly suggest a plugin etc or a methodology to solve the problem?
>
> Thanks and Best
> FlyGuy
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> ***************************
> With thanks and Best Wishes
> Fly Guy
> Twitter: @flyguy
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