Posted by
Herbie on
URL: http://imagej.273.s1.nabble.com/Calculating-grazing-area-tp5019047p5019048.html
Good day Josh,
you've realized the main problem with threshold-based binarization...
Although there are equalization methods to help with uneven signals,
they won't do any magic.
Try to find a solution without thresholds:
Perhaps classification may help or a more adequate way of image
acquisition (often not considered!).
In any case, without seeing a _typical_ example image, it is difficult
to give more helpful advice.
Best
Herbie
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Am 10.07.17 um 15:53 schrieb joshcurran:
> Hi there,
>
> I'm brand new to ImageJ and am just trying to get to grips with it. I've
> been playing around with it all day.
>
> I'd like to calculate the percentage area grazed by snails across an algal
> biofilm. I understand that I can threshold the image, but the biofilm isn't
> amazingly consistent with colour across the dish. When I was practicing and
> testing it out earlier on when thresholding it wasn't really covering the
> parts I wanted it to. Any advice or tips? Or is there another way that
> grazing area could be measure?
>
> Many thanks,
> Josh
>
>
>
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