Re: Calculating grazing area

Posted by jerie on Jul 11, 2017; 4:35pm
URL: http://imagej.273.s1.nabble.com/Calculating-grazing-area-tp5019047p5019052.html

Dear Josh,

please post an image; your issues with segmentation may result from
illumination artefacts; eg. you would initially try to generate images with
good contrast, because issues during segmentation or classification
oftentimes result from low or uneven contrast.

Kind regards, Jens

Dr. Jens Rietdorf, visiting scientist @ center for technological
development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de Janeiro
Brasil.

On Mon, Jul 10, 2017 at 10:53 AM, joshcurran <[hidden email]> wrote:

> Hi there,
>
> I'm brand new to ImageJ and am just trying to get to grips with it. I've
> been playing around with it all day.
>
> I'd like to calculate the percentage area grazed by snails across an algal
> biofilm. I understand that I can threshold the image, but the biofilm isn't
> amazingly consistent with colour across the dish. When I was practicing and
> testing it out earlier on when thresholding it wasn't really covering the
> parts I wanted it to. Any advice or tips? Or is there another way that
> grazing area could be measure?
>
> Many thanks,
> Josh
>
>
>
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