RGB tiff saving as Composite
Posted by
Glen MacDonald-2 on
Jul 14, 2017; 6:49pm
URL: http://imagej.273.s1.nabble.com/RGB-tiff-saving-as-Composite-tp5019065.html
My macro stitches z-series confocal images into a montage, all as composite images. The macro converts the resulting montage into an RGB.
but, saving the image writes a composite image. I can pause the montage and verify that it is a RGB montage stack with a header info that says “RGB" , but, manually saving it still creates a composite! I’m pretty sure this used to work.
Is this a bug or am I overlooking something? I”m pretty certain this used to work.
Create montage code...
Composite to RGB conversion:
selectWindow("montage");
run("Arrange Channels...", "new=321");
Stack.setDisplayMode("color");
Stack.setChannel(1);
run("Red");
Stack.setChannel(2);
run("Green");
Stack.setChannel(3);
run("Blue");
//Stack.setChannel(4);
//run("Grays");
Stack.setDisplayMode("composite");
run("Stack to RGB", "slices");
Save the file:
saveAs("tiff",target+sep+dirname);
Thanks,
Glen MacDonald
Digital Microscopy Center
Box 357923
University of Washington
Seattle, WA 98195-7923 USA
(206) 616-4156
depts.washington.edu/digmicro
[hidden email]
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