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Counting OVERLAPPING cells (Watershed not accurate)

Posted by anne on Jul 15, 2017; 6:53pm
URL: http://imagej.273.s1.nabble.com/Counting-OVERLAPPING-cells-Watershed-not-accurate-tp5019069.html




Hi!

I am trying to analyze this image using ImageJ. I want ImageJ to count the stained cells. However, due to overlap of multiple cells, when I make the image binary I lose the original boundaries and hence, ImageJ is unable to watershed correctly. Also, seems watershed only works well with circular objects.

I used Process --> Find edges which outlines the cells relatively more accurately. To analyze particles, I would have to make image binary or threshold and in both circumstances the cluster of cells is counted as 1 cell by 'Analyze Particles'.

Any advice on how to count cells would be greatly appreciated!
Thanks!
A