Re: 3D Suite - 3D Distance map, file input bug?

Posted by Thomas Boudier-4 on Jul 20, 2017; 2:17am
URL: http://imagej.273.s1.nabble.com/3D-Suite-3D-Distance-map-file-input-bug-tp5019093p5019095.html

Hi Matt,

The problem is the calibration of the image, for the "good" one it´s
0.06x0.06x0.4 microns and for the "bad" one it´s 0.16x0.16x0.16, so it
looks like the Z calibration is lost for the "bad" one. You can easily
check if the calibration is correct by visualising your data in 3D viewer .

Best,

Thomas

On 19/07/2017 23:57, Matthew Jones wrote:

> Hi,
>
> Apologies if my last email was mangled - the text seemed to disappear.
>
> I get different results depending on which image I process with the 3D distance map plugin. The metadata for the two files is different, however I don’t know when this difference originated (possibly when using an older version of imageJ?).
>
> Is anyone able to tell what is different about the images from the metadata and how to convert from ‘bad’ to ‘good’ format.
>
> Also, a big thank you to Thomas Boudier for 3D Suite and related plugins as they have been invaluable in my research and generally work excellently!
>
> Kind regards,
>
> Matt
>
> p.s. my system is  Macbook Pro OS X Yosemite 10.10.3 and FIJI (though some of the images may have been processed in an older version of ImageJ at an earlier stage).
> original files at: https://drive.google.com/open?id=0B_9C1WFBZYoUbzRBVE5nUXJrWDg
>
> --
> ImageJ mailing list: http://imagej.nih.gov/ij/list.html

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       Thomas Boudier, Associate Professor, UPMC,
       Université Pierre et Marie Curie, Paris, France.
       BioInformatics Institute (BII)/IPAL, Singapore.
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