Posted by
Jacob Keller-2 on
Aug 23, 2017; 11:05pm
URL: http://imagej.273.s1.nabble.com/Image5D-bug-tp5019256.html
I have been using the nice capabilities of image5D, but there seem to be a
couple of problems. My procedure is to split multichannel .czi xyzct stacks
into individual channels, stack-to-image5D, then montage. I noticed some
strange behaviors in so doing:
-when I make a montage, it is output as RGB, even though the input is 8- or
16-bit. Not too terrible, since it's easy enough to convert to 8-bit from
there, although it's a shame to lose the 16-bit depth, and it seems like
something funky is going on.
-the last frame in the timelapse montages are always black
-Contrast is weird: when I do not click "copy contrast" the images are
output way too bright, and it's not just the contrast setting. When I do
click "copy contrast," sometimes things are fine, sometimes the intensities
(not just contrast settings) make discrete jumps inexplicably.
Can you help me out here?
Jacob Keller
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