> I get the error msg "5D hyperstacks are not supported." Pls find attached
> MCVE (same as other post.) This also happens when I get rid of one channel,
> so it's only 4D.
>
> Jacob
>
> On Thu, Aug 24, 2017 at 10:49 AM, Curtis Rueden <
[hidden email]> wrote:
>
> > Hi Jacob,
> >
> > The Image5D plugin, while currently part of Fiji, is not actively
> supported
> > [1].
> >
> > Is there some reason that ImageJ's built-in support for 5D hyperstacks,
> > along with the "Make Montage..." command, cannot accomplish what you
> need?
> >
> > The following macro creates a 16-bit montage of two images without using
> > Image5D:
> >
> > run("Blobs (25K)");
> > run("16-bit");
> > run("Clown (14K)");
> > run("16-bit");
> > run("Images to Stack", "method=[Copy (center)] name=Stack title=[] use");
> > run("Make Montage...", "columns=2 rows=1 scale=1");
> >
> > Regards,
> > Curtis
> >
> > [1]
http://imagej.net/Image5D; see right-hand sidebar
> >
> > --
> > Curtis Rueden
> > LOCI software architect -
https://loci.wisc.edu/software> > ImageJ2 lead, Fiji maintainer -
https://imagej.net/User:Rueden> > Did you know ImageJ has a forum?
http://forum.imagej.net/> >
> >
> > On Wed, Aug 23, 2017 at 6:05 PM, Jacob Keller <
[hidden email]>
> > wrote:
> >
> > > I have been using the nice capabilities of image5D, but there seem to
> be
> > a
> > > couple of problems. My procedure is to split multichannel .czi xyzct
> > stacks
> > > into individual channels, stack-to-image5D, then montage. I noticed
> some
> > > strange behaviors in so doing:
> > >
> > > -when I make a montage, it is output as RGB, even though the input is
> 8-
> > or
> > > 16-bit. Not too terrible, since it's easy enough to convert to 8-bit
> from
> > > there, although it's a shame to lose the 16-bit depth, and it seems
> like
> > > something funky is going on.
> > >
> > > -the last frame in the timelapse montages are always black
> > >
> > > -Contrast is weird: when I do not click "copy contrast" the images are
> > > output way too bright, and it's not just the contrast setting. When I
> do
> > > click "copy contrast," sometimes things are fine, sometimes the
> > intensities
> > > (not just contrast settings) make discrete jumps inexplicably.
> > >
> > > Can you help me out here?
> > >
> > > Jacob Keller
> > >
> > > --
> > > ImageJ mailing list:
http://imagej.nih.gov/ij/list.html> > >
> >
> > --
> > ImageJ mailing list:
http://imagej.nih.gov/ij/list.html> >
>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>