Re: Oil red O

Posted by Daan VAN DEN BRINK on Aug 30, 2017; 11:24am
URL: http://imagej.273.s1.nabble.com/Oil-red-O-tp5019290p5019296.html

Goedemiddag,

I have some experience quantifying lipid stains in biological samples
using ImageJ, and usually calculate %area covered by staining, along the
line of what Adrián suggests. To analyze my samples I have made a script
that does the business. Maybe you could attach a sample image to show us
precisely what you need?

Daan

ps. in my experience Oil Red O fades very quickly which might make
quantification difficult.

On 29/08/17 16:08, Adrián Villalba wrote:

> Dear Prof. Van den Broeck,
>
> Which is exactly what you want to analyze - or the expected output -? If
> you want to quantify the number of particles stained in Oil red, then maybe
> you can try to Threshold (Adjust-Threshold) and then Analyze-Particle
> analizer, playing with the different min/max values in the thresholding.
>
> All my best,
>
> 2017-08-29 15:47 GMT+02:00 Wim Van Den Broeck <[hidden email]>:
>
>> Dear friends,
>>
>> I am just starting using Image J, so no experience at all.
>>
>> However, I do have a very particular question. I need to analyse
>> cryosections stained with Oil Red O, so is there any Oil red O plugin
>> available ?
>>
>> Thanks a lot for your answers / help.
>>
>> Kind regards,
>>
>> Wim Van den Broeck.
>>
>> [cid:image001.png@01D21591.203AEEA0]
>>
>>
>>
>>
>>
>>
>> Prof. dr. Wim Van den Broeck, DVM, MSc, PhD
>> Cell Biology and Histology
>> T [phone]
>>
>> Department of Morphology
>> Faculty of Veterinary Medicine, D5, Salisburylaan 133, 9820 Merelbeke,
>> BELGIUM
>> T [phone] / F [phone]
>>
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>

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