Re: Background correction

Posted by Jeremy Adler-2 on Sep 30, 2017; 2:56pm
URL: http://imagej.273.s1.nabble.com/Background-correction-tp5019468p5019469.html

My immediate thought would be to add a physical filter to the microscope to reject reflected light.

-----Original Message-----
From: ImageJ Interest Group [mailto:[hidden email]] On Behalf Of anusuya pal
Sent: 30 September 2017 16:11
To: [hidden email]
Subject: Background correction

Dear all,

I have image which has the background issue due to the reflected light from the sample. To understand whether the mean intensity varies actually due to sample configuration, I need to first correct the background. Ideally the mean intensity should be zero as the image is 8 bit with dark background.
But it is not the case for my sample.

I know there are various techniques which correct the background issue in stacks. I have an idea about only one technique, called BaSiC. Is there any other way to correct the background in stack?

My current image is not in stack, it is a single image. May I know whether there is any tool for background correction for single image?

Any suggestions?

Thanks
Anu

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