Eastimation signal to noise ratio on imageJ

Posted by thpnick688 on Oct 22, 2017; 3:18pm
URL: http://imagej.273.s1.nabble.com/Eastimation-signal-to-noise-ratio-on-imageJ-tp5019580.html

Hi, everyone,
     I have encountered a question on estimating signal to noise ratio on
imageJ. I have an image with 4x4 binning. The binning is done by the CCD
detector not by the software. I use the ROI to measure the mean gray level
intensity on the main object in the image. I also use the same size ROI on
the background to measure the background noise. I use the simple equation
"S/N= “mean gray level intensity in subject” / “mean gray level intensity of
the noise”" to estimate the signal to noise ratio. However, by comparing
with an image that is unbinned, I didn't see the SNR is improved after the
calculation. So, what mistakes did I make?

RL



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