http://imagej.273.s1.nabble.com/Marking-areas-of-like-pixel-values-tp5019664p5019668.html
The .fit format allows 16-bit images. One might assume that a camera producing 12-bit images
> On Nov 6, 2017, at 10:57, Gabriel Landini <
[hidden email]> wrote:
>
> On Monday, 6 November 2017 16:33:39 GMT
[hidden email] wrote:
>> I am taking pictures with a 12 bit camera in .FIT file format. When I open
>> them in Fiji it says they are 32 bit and therefore it won't perform auto
>> thresholding on them because it says this only works for 8 or 16 bit
>> images. Why and how does it change my 12 bit image into something that
>> it is not?
>
> I think you might be trying the Auto Threshold or Auto Local Threshold
> commands. Those do not work on 32 bit images.
> You could try the original Adjust Threshold command, that would rebin the
> image into a smaller greyscale space and threshold it.
>
> As to your other question, I have no idea. There are no 12 bit images in IJ,
> so perhaps the importer, as a safety measure, might be opening them as 32bit.
> Cheers
>
> Gabriel
>
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