Using 'analyse particles' on stacks: compensating for multiple counting in adjacent slices
Posted by
Alex Lamb on
Jan 08, 2018; 6:36pm
URL: http://imagej.273.s1.nabble.com/Using-analyse-particles-on-stacks-compensating-for-multiple-counting-in-adjacent-slices-tp5019844.html
Hello!
I've been using the 'analyse particles' tool on stacks, processing each slice separately. This has been working fine except that since the particles of interests are 3 dimensional they are often present in multiple neighbouring slices, so are counted multiple times.
I know that the output of the 'Stack Particle Analysis' plugin gives x,y co-ordinates of each particle identified by the tool and I was wondering if it could be possible to use these co-ordinates to discount particles that occupy the same position in adjacent slices as a way of stopping particles being counted mutliple times in neighbouring slices.
If not then is there an alternative method or plugin that will do this?
Thanks a lot!
Alex
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