Find missing spots in a grid of spots

Posted by Cornelius Sicker-2 on
URL: http://imagej.273.s1.nabble.com/Find-missing-spots-in-a-grid-of-spots-tp5019879.html

Dear fellow ImageJ users,

a colleague of mine is looking for an automated solution to find missing
spots in a rectangular grid of small spots. The spots are quite
uniformly sized and have similar brightness. The grid might be rotated
slightly. Only a few spots are expected to be missing in each picture
(<1 % of all grid positions). The grid itself is only virtually created
by the visible spots. Apart from the visible spots on dark background
there are no distinguishing features in the images. There are thousands
of images to be processed, so the solution has to be fully automated.

I think it should be not hard to find the coordinates of the existing
spots by some particle analysis plugins in ImageJ. However, given a list
of coordinates of visible spots, how would you identify the missing
ones? Does anybody know of an existing solution to solve this?

I can provide an example image if it helps, but was not sure how the
mailing list policy is about attachments.

Best regards
Cornelius

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