Posted by
Jeremy Adler-2 on
URL: http://imagej.273.s1.nabble.com/Find-missing-spots-in-a-grid-of-spots-tp5019879p5019882.html
To find the missing spots,
Create a binary image of the spots you have - thresholding
Create a distance map outwards from the spots.
Locations of 'Missing spots' will be shown in the distance map by thresholding -
-----Original Message-----
From: ImageJ Interest Group [mailto:
[hidden email]] On Behalf Of Cornelius Sicker
Sent: den 14 januari 2018 19:36
To:
[hidden email]
Subject: Find missing spots in a grid of spots
Dear fellow ImageJ users,
a colleague of mine is looking for an automated solution to find missing spots in a rectangular grid of small spots. The spots are quite uniformly sized and have similar brightness. The grid might be rotated slightly. Only a few spots are expected to be missing in each picture
(<1 % of all grid positions). The grid itself is only virtually created by the visible spots. Apart from the visible spots on dark background there are no distinguishing features in the images. There are thousands of images to be processed, so the solution has to be fully automated.
I think it should be not hard to find the coordinates of the existing spots by some particle analysis plugins in ImageJ. However, given a list of coordinates of visible spots, how would you identify the missing ones? Does anybody know of an existing solution to solve this?
I can provide an example image if it helps, but was not sure how the mailing list policy is about attachments.
Best regards
Cornelius
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