registration of two channels with beads reference help please

Posted by Cammer, Michael on Jan 23, 2018; 7:42pm
URL: http://imagej.273.s1.nabble.com/registration-of-two-channels-with-beads-reference-help-please-tp5019938.html

We are looking for a solution to a registration problem and would appreciate help.

At the beginning of each timelapse sequence, we take images of multicolor beads as a reference.  We would like to have the reference image registered and apply the parameters for the registration to about 20 other 2 channel timelapse files.  We expect that we would need five points to do the correction, one from each corner and one from the center.  The initial registration could involve the user clicking on coincident spots or could be automatic.  While we would prefer to keep the original 16 bit depth, we could make do with 8 bits.

An example beads picture is at http://microscopynotes.com/temp/AVG_20171214_beads.tif  (In the future we will try to use smaller beads.)

Any help appreciated.

Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY  10016
C: 914-309-3270  [hidden email]<mailto:[hidden email]>  http://nyulmc.org/micros  http://microscopynotes.com/



------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
=================================

--
ImageJ mailing list: http://imagej.nih.gov/ij/list.html