Re: Find missing spots in a grid of spots

Posted by ard on
URL: http://imagej.273.s1.nabble.com/Find-missing-spots-in-a-grid-of-spots-tp5019879p5019964.html

On 2018/01/31, at 06:00, IMAGEJ automatic digest system <[hidden email]> wrote:
> We take X and the Y projections for the image and use a
> standard peak detecting algorithm to generate a list of peaks which
> should correspond to the positions of the columns (X), and rows (Y).

We try to find in a similar way all spot locations of a hexagonal grid in 1024x1024px images, with the same characteristics of missing spots as those in the original question.
The web search for X-project or Y-project does not really return useful results because in image processing they are too common a term.

As 'Scale' to 1x1024 and 1024x1 images does not allow for min/max/mean/modal/stdev like z-project does it misses a lot of more than obvious spots. The 'simplest' alternative we could find was to duplicate an image into a 1024x1024x2 stack, do a reslice to 2x1024x1024 and 1024x2x1024, then do a z-project 'Sum' to a 2x1024 or a 1024x2 image and located maxima in these images.


Is there a command which I overlooked, which is equivalent to the 'Z-project', that can project the plane in X or Y direction onto a single pixel wide image?

Ard
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