Re: Find missing spots in a grid of spots

Posted by ard on
URL: http://imagej.273.s1.nabble.com/Find-missing-spots-in-a-grid-of-spots-tp5019879p5019987.html

@ Gabriel,

In this particular case, a 'nice' image, indeed the blob is morphologically
different from the dots. Many of the images have much more deteriorated
dots, down to a dozen of pixels, and blobs and dots are hard to separate,
except for the regularity in their x- and y coordinate increments. We
measure the x-y centers of gravity and then reconstruct the original grid by
drawing circles of known size to be used as a mask. Having all centers gives
a more robust reconstruction of the mask.

@ Herby,
I did not know that plugin yet and will experiment with it. The result of
your attempt looks promising. When I can find all dots as clearly
outstanding from the background as your result, it is easy to find them via
particle analysis and thus locate the centers. Thanks for pointing me in
that direction.

Hoping Cornelius also benefits form these answers, I'm off to implement :-)

Ard



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