Re: Extended Particle Analyzer - Different results on PC and Mac

Posted by gankaku on Feb 06, 2018; 8:34am
URL: http://imagej.273.s1.nabble.com/Extended-Particle-Analyzer-Different-results-on-PC-and-Mac-tp5019990p5020004.html

 Dear Volko,

Can you provide the complete macro?
My guess would be the black background definition (except the correct
particles are selected by the ROIs) or as Aryeh already stated any of the
other parameters. I personally cannot test it on a mac but have not seen
this behaviour before.

Regards,
Jan


2018-02-05 15:42 GMT+01:00 Straub, Volko A. (Dr.) <[hidden email]>:

> Dear All,
>
> I have observed some unexpected behaviour using the Extended Particle
> Analyzer in a macro.
> I have analysed the attached image using the Extended Particle Analyzer as
> part of a macro with the following command:
>
> min=20;
> max=300;
> run("Extended Particle Analyzer", "area="+min+"-"+max+" pixel
> roundness=0.4-1.00 show=Nothing redirect=None keep=None add reset");
>
> This generates a list of 507 ROIs in the ROI manager.
>
> However, when I gave the macro to a student for some analysis, who is
> using a Mac laptop, using the same command on the same image only found 287
> ROIs. Manual inspection of the ROI table showed that many of the larger
> particles were missed.
> I am stumped and have no idea why the same macro command applied to the
> same image should produce such different results just because the macro is
> executed on a different operating system.
>
> Is this a bug? Any ideas what might be going on?
> Thanks,
> Volko
>
>
>
>
>
>
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