Posted by
csadangi on
Feb 06, 2018; 5:43pm
URL: http://imagej.273.s1.nabble.com/Splitting-images-Histograms-tp5019997p5020015.html
Hi,
1)It is not going to affect the definition of the image
2 and 4) Select Open Files individually. Because of the Memory of your
computer I'm worried that you will not be able to open all 30 series in the
same time. Once you have the second "Bio-Formats Series Options" window,
select the series you want to open at once.
3)I'm not having this issue with the SP5. In the color mode you might try
"composite" or "colorized". With the composite you can use the "channels
tool" to have one or several channels displayed in the same time.
5)They are just showing a line plot. The two pics correspond to the
Intensity at the two opposite side of the cell. They draw two plot profiles
(can be sequential) and get the value of the peak, then average all 4 peak
values. (1- Draw a line crossing the cell, then go to Analyze/Plot Profile.
On the new window, get the "list" and you can export the intensity values
to Excel.
In Excel find the two highest values per line.
You can obviously create a Macro to automate all these steps.
-----------------------------------
When I open the files on Windows computer, I have the problem that it shows
only one colour. But if I open the files in Mac, then I can see the three
different colours.
5) How can I have the lines drawn like in the paper? What should be done
after I find the two highest values?
Thanks.
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