Posted by
Chloe van Oostende on
Feb 07, 2018; 4:04am
URL: http://imagej.273.s1.nabble.com/Splitting-images-Histograms-tp5019997p5020019.html
Hi,
The two highest values are your plasma membrane fluorescent intensity, so
you average them. Calculate several cells and you can after get
statistically relevant data.
I bet that what they draw in the paper fig 6S1A, is not from Fiji. They
just made a nice figure, didactic.
What you care about are the data from the "Plot profile" tool in Fiji.
To make as nice as Fig 6S1A, use Powerpoint or Illustrator in order to
explain what you are doing.
Best,
Chloe
*Chloë van Oostende, PhD*
Chair,* Plant Science* session
Canadian Microscopy and Cytometry Symposium 2017
Facility manager- Senior Microscopy specialist
*Cell Biology and Image Acquisition Core Facility*
Faculty of Medicine
University of Ottawa
RGN 3171
451 Smyth Road
Ottawa, ON K1H 8M5
Office: 613-562-5800 ext: 8376
http://www.chloevanoostende.netCBIA core facility
<
http://www.med.uottawa.ca/research/corelabs/CoreLabs_CBIA/eng/>
2018-02-06 12:43 GMT-05:00 csadangi <
[hidden email]>:
> Hi,
> 1)It is not going to affect the definition of the image
> 2 and 4) Select Open Files individually. Because of the Memory of your
> computer I'm worried that you will not be able to open all 30 series in the
> same time. Once you have the second "Bio-Formats Series Options" window,
> select the series you want to open at once.
> 3)I'm not having this issue with the SP5. In the color mode you might try
> "composite" or "colorized". With the composite you can use the "channels
> tool" to have one or several channels displayed in the same time.
> 5)They are just showing a line plot. The two pics correspond to the
> Intensity at the two opposite side of the cell. They draw two plot profiles
> (can be sequential) and get the value of the peak, then average all 4 peak
> values. (1- Draw a line crossing the cell, then go to Analyze/Plot Profile.
> On the new window, get the "list" and you can export the intensity values
> to Excel.
> In Excel find the two highest values per line.
>
> You can obviously create a Macro to automate all these steps.
> -----------------------------------
> When I open the files on Windows computer, I have the problem that it shows
> only one colour. But if I open the files in Mac, then I can see the three
> different colours.
>
> 5) How can I have the lines drawn like in the paper? What should be done
> after I find the two highest values?
>
> Thanks.
>
>
>
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