Re: Splitting images & Histograms

Posted by csadangi on Feb 08, 2018; 1:03am
URL: http://imagej.273.s1.nabble.com/Splitting-images-Histograms-tp5019997p5020026.html

I tried what you suggested. First I drew a Vertical line and measured the
plot profile. I exported it to an excel sheet and looked for the two highest
values:
X = 0.848; y = 141.41 - vertical
X = 3.39; y = 106.44

I repeated the same step by drawing a horizontal line
X = 1.13; Y = 60,77 - Horizontal
X = 3.10; Y = 106.14

I averaged all the Y values and got 103.69. Can I mention this value as an
arbitrary unit (A.U.) as they do it in the paper? How can I define an
arbitrary unit? Also, do you suggest doing similar calculations for a number
of other cells and then doing statistics? In the paper they say "The peak
values of each line (2 values/line) were used to calculate peak fluorescent
intensity of KCC2 at the membrane". So, can I say the peak fluorescent
intensity of the membrane is 103.69 au

Another doubt I have is that I cannot change the X-axis to log value as they
show it in the paper. I changed the X-axis from Set Range option to display
as log value, but it didn't change and it still shows in microns.



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