http://imagej.273.s1.nabble.com/Voronoi-plugin-not-adjusted-tp5020029p5020033.html
I usually do this sort of restriction by limiting the analysis to Voronoi regions which are completely in the ROI (your "green region"). But, I also always roll my own Voronoi computations, and have no clue how to do that using the standard tool, and especially not in the macro language.
You WANT the Voronoi computation to include the entire image, because those half-infinite regions provide boundaries for the ones you want to analyze. Use as much area as possible to create the Voronoi Diagram, and then clip out and reject any Voronoi region that is not entirely contained inside your ROI. In practice, this means computing the Voronoi vertices and checking that each one is inside the ROI,
> On 8 Feb 2018, at 10:08 , Adrián Villalba <
[hidden email]> wrote:
>
> Dear all,
>
> Thank you all for your help. I am trying to perform Voronoi - using the
> DelaunayVoronoi plugin in Plugin>Analyze) to calculte (aproximately) the
> area of positive staining in each cell.
>
> I used a homemade macro:
>
> run("Duplicate...", " ");
> title = getTitle();
> run("Split Channels");
>
> selectWindow(title+" (green)");
> // process the green channel;
> setAutoThreshold("Moments dark");
> //run("Threshold...");
> //setThreshold(69, 255);
> setOption("BlackBackground", false);
> run("Convert to Mask");
> run("Close-");
> run("Fill Holes");
> run("Dilate");
> run("Remove Outliers...", "radius=50 threshold=50 which=Bright");
> run("Create Selection");
>
>
> selectWindow(title+" (blue)");
> // process the blue channel;
> run("Restore Selection");
> run("Unsharp Mask...", "radius=5 mask=0.90");
> run("Remove Outliers...", "radius=3 threshold=2 which=Bright");
> run("Find Maxima...", "noise=50 output=[Maxima Within Tolerance]");
> run("Delaunay Voronoi", "mode=Voronoi interactive");
> run("Close-");
> run("Fill Holes");
> run("Watershed");
> run("Find Maxima...", "noise=1 output=[Point Selection] light");
> run("Delaunay Voronoi", "mode=Voronoi interactive");
>
>
> The point is that the surrounding nuclei of my section has a Voronoi area
> to the boundaries of the image and i only want to perform that in the
> green-positive selection. I can not restore in the same image the find
> maxima output to locate the nuclei and the selection i previously used in
> my green channel. Does anyone know how can i apply Voronoi with my
> selection boundaries instead of performing Voroni to the whole picture? And
> if it is possible, how can i measure the area of each Voronoi-region?
>
> Thank you in advance!
>
>
> --
>
> - Adrián Villalba Felipe.
>
https://es.linkedin.com/in/adrianvillalba>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html> <input_Voronoi.tif><Voronoi output.tif>