http://imagej.273.s1.nabble.com/Finding-the-regions-automatically-tp5020109p5020136.html
Process>Binary>Watershed. It will create some extra lines from the
> Hello everyone,
>
> Thanks for giving me different suggestions.
>
> Replying to Michael's question, Can't you simply run 'Analyze
> Particles'? I
> think I can't just do it, to get the accurate results, I need to do
> some
> work before we just use 'analyze particles'. If I do so on the raw
> image,
> there are some lines which are not perfectly continuous, there are
> gaps,
> and if I just do analyze particles, it takes some of the gaps as
> particles
> and count the area, which I think is not correct. However I did this
> and
> got sum of the areas as 499130 pixel^2 with counts 633.
>
> Replying to Cammer's suggestion, Threshold, Erode,Analyze, if I erode,
> yes,
> the lines will be perfectly continuous, but the lines become thicker, I
> should do to make those lines thin again, otherwise the areas of the
> domains are not perfect. However, I did this, I got the count as 211
> and
> sum as 580561.
>
> Now, comes to Herbie's and Jeremy's approaches. Herbie's approach gives
> counts as 207 with sum as 425532 and Jeremy's macro gives 186 with
> 495383
> pixel^2. The image has so many domains that I can't check manually, may
> I
> know what to do? Is there any way to check that the total area of
> domains
> are almost perfect?
>
> Thanks
> Anu
>
>
>
>
>
>
> On Fri, Feb 16, 2018 at 10:00 AM, Cammer, Michael <
>
[hidden email]> wrote:
>
>> Theshold
>> Erode
>> Analyze particles
>>
>> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York,
>> NY
>> 10016
>> C: 914-309-3270
[hidden email] http://nyulmc.org/micros>>
http://microscopynotes.com/>>
>>
>>
>> -----Original Message-----
>> From: anusuya pal [mailto:
[hidden email]]
>> Sent: Thursday, February 15, 2018 5:34 PM
>> To:
[hidden email]
>> Subject: Finding the regions automatically
>>
>> Hello,
>>
>> Greetings of the day!
>>
>> I have a binary image, and want to find the area of each of the
>> domains. I
>> can do that with nearest neighbor tool by selecting each domain and to
>> add
>> in roiManager, and then to measure the area of each domain.
>>
>> I want to know is there any other way so that the selection of the
>> domains
>> can be done automatically?
>>
>> For convenience, attaching the binary image (a_75) and the output
>> image
>> obtained from nearest neighbor tool showing one domain in red color.
>> Your
>> help will be appreciated!
>>
>> Thanks in advance!
>> Anu
>>
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