http://imagej.273.s1.nabble.com/Finding-the-regions-automatically-tp5020109p5020137.html
> Hi Anu,
>
> if you want to close the gaps in the lines, you can try
> Process>Binary>Watershed. It will create some extra lines from the
> cricumference to the border, but they should not hurt.
>
> Of course, this will change the number of total pixels (the newly drawn
> lines won't be part of your domains any more), but if the missing line
> segments were ther, it would probably change the number of pixels even more.
>
>
> Michael
> ________________________________________________________________
>
>
>
> On 2018-02-17 02:56, anusuya pal wrote:
>
>> Hello everyone,
>>
>> Thanks for giving me different suggestions.
>>
>> Replying to Michael's question, Can't you simply run 'Analyze Particles'?
>> I
>> think I can't just do it, to get the accurate results, I need to do some
>> work before we just use 'analyze particles'. If I do so on the raw image,
>> there are some lines which are not perfectly continuous, there are gaps,
>> and if I just do analyze particles, it takes some of the gaps as particles
>> and count the area, which I think is not correct. However I did this and
>> got sum of the areas as 499130 pixel^2 with counts 633.
>>
>> Replying to Cammer's suggestion, Threshold, Erode,Analyze, if I erode,
>> yes,
>> the lines will be perfectly continuous, but the lines become thicker, I
>> should do to make those lines thin again, otherwise the areas of the
>> domains are not perfect. However, I did this, I got the count as 211 and
>> sum as 580561.
>>
>> Now, comes to Herbie's and Jeremy's approaches. Herbie's approach gives
>> counts as 207 with sum as 425532 and Jeremy's macro gives 186 with 495383
>> pixel^2. The image has so many domains that I can't check manually, may I
>> know what to do? Is there any way to check that the total area of domains
>> are almost perfect?
>>
>> Thanks
>> Anu
>>
>>
>>
>>
>>
>>
>> On Fri, Feb 16, 2018 at 10:00 AM, Cammer, Michael <
>>
[hidden email]> wrote:
>>
>> Theshold
>>> Erode
>>> Analyze particles
>>>
>>> Michael Cammer, Sr Research Scientist, DART Microscopy Laboratory
>>> NYU Langone Health, 540 First Avenue, SK2 Microscopy Suite, New York, NY
>>> 10016
>>> C: 914-309-3270
[hidden email] http://nyulmc.org/micros>>>
http://microscopynotes.com/>>>
>>>
>>>
>>> -----Original Message-----
>>> From: anusuya pal [mailto:
[hidden email]]
>>> Sent: Thursday, February 15, 2018 5:34 PM
>>> To:
[hidden email]
>>> Subject: Finding the regions automatically
>>>
>>> Hello,
>>>
>>> Greetings of the day!
>>>
>>> I have a binary image, and want to find the area of each of the domains.
>>> I
>>> can do that with nearest neighbor tool by selecting each domain and to
>>> add
>>> in roiManager, and then to measure the area of each domain.
>>>
>>> I want to know is there any other way so that the selection of the
>>> domains
>>> can be done automatically?
>>>
>>> For convenience, attaching the binary image (a_75) and the output image
>>> obtained from nearest neighbor tool showing one domain in red color. Your
>>> help will be appreciated!
>>>
>>> Thanks in advance!
>>> Anu
>>>
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