Posted by
Stoyan Pavlov on
URL: http://imagej.273.s1.nabble.com/How-to-count-the-number-of-pixels-over-certain-grayscale-value-in-a-ROI-tp5020254p5020271.html
In addition to to Kees' answer above: the "Limit to threshold" box must
also be ticked in "Set measurements..." dialog.
Good day,
Stoyan
---
Assoc.Prof. Stoyan P. Pavlov, MD, PhD
Departament of Anatomy, Histology and Embryology
Medical University "Prof. Dr. Paraskev Stoyanov", Varna
Prof. Marin Drinov Str.55
9002 Varna
Bulgaria
Tel: +359 (0) 52 - 677 - 052
e-mail:
[hidden email]
[hidden email]
2018-03-12 10:47 GMT+02:00 Straatman, Kees (Dr.) <
[hidden email]>:
> Set the threshold values using Image > Adjust > Threshold and add your
> ROI, go to Analyze > Set Measurements... and select 'Area' followed by
> Analyze > Analyze Particles with 'Summary' selected you get the number of
> pixels under total Area. If your image is scaled also select 'Pixel units'
> in Analyze Particles.
>
> Best wishes
>
> Kees
>
>
> Dr Ir K.R. Straatman
> Senior Experimental Officer
> Advanced Imaging Facility
> Centre for Core Biotechnology Services
> University of Leicester
> www.le.ac.uk/advanced-imaging-facility
>
>
>
> -----Original Message-----
> From: Jiseok [mailto:
[hidden email]]
> Sent: 09 March 2018 22:22
> To:
[hidden email]
> Subject: How to count the number of pixels over certain grayscale value in
> a ROI?
>
> Hi, I am trying to count the number of pixels in a ROI which have
> grayscale value over a certain threshold (for example, if the range is
> 0-255, count the number of pixels with values over 30). Is there a simple
> way to do this?
> I don't know how to write or edit plugins or macros...
>
> Thank you.
>
>
>
> --
> Sent from:
http://imagej.1557.x6.nabble.com/>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>
> --
> ImageJ mailing list:
http://imagej.nih.gov/ij/list.html>
--
ImageJ mailing list:
http://imagej.nih.gov/ij/list.html