Re: Issues with Analyse Particule

Posted by Gabriel Landini on Apr 18, 2018; 3:21pm
URL: http://imagej.273.s1.nabble.com/Issues-with-Analyse-Particule-tp5020527p5020529.html

On Wednesday, 18 April 2018 15:43:01 BST [hidden email] wrote:

> To start, I think to have found a bug (using the daily built version of
> ImageJ 1.52a57) where the objects found with the "Analyze Particles..." tool
> are not really matching with the threshold when applied to the following
> picture:
>
>  <https://seafile.unistra.fr/d/f8f47d8fbdf84c098971/>
> https://seafile.unistra.fr/d/f8f47d8fbdf84c098971/
> by using the following macro code :
> setAutoThreshold("MinError dark");
> run("Analyze Particles...", "size=0-100 clear add");
> waitForUser("a");
> run("Analyze Particles...", "size=0-Infinity clear add");

The problem here is that you are using a 16bit image and the threshold is
shown on a 16bit overlay.
To see what you are exactly analysing, please binarise the image and then
apply the particle analysis.

Cheers

Gabriel

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