Re: Stitching together multiple images within a series of folders

Posted by Stephan.Preibisch@mdc-berlin.de on
URL: http://imagej.273.s1.nabble.com/Stitching-together-multiple-images-within-a-series-of-folders-tp5020586p5020589.html

Hi,

You can define many types of grids before starting the stitching, and it does support C. elegans image stitching. This is one of the cases we originally developed it for :) We also wrote quite extensive documentation, did you read it on how to set up grids? It is pretty straight forward:

http://imagej.net/Image_Stitching … specifically here: http://imagej.net/Image_Stitching#Grid.2FCollection_Stitching - you can choose from many grid types, and a simple line can represented by some of them

We also just finished the BigStitcher which is a significant extension of the Stitching plugins. One feature that might specifically be interesting for you is that you can interactively try different grid types, so you can adjust sliders until the initialization is right by real-time display of the result. You can find general instructions here: http://imagej.net/BigStitcher … Again, specifically on the interactive grid initialization here: http://imagej.net/BigStitcher_manual_translation#Move_Tiles_To_Regular_Grid_…

At the end of the day, both are macro-scriptable, so once you know the basic workflow it is easy to automate.

All the best,
Stephan
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Dr. Stephan Preibisch
Group Leader

Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC)
Building 89, 1.08b
Robert-Rössle-Str. 10
13125 Berlin

email: [hidden email]<mailto:[hidden email]>
web: http://preibischlab.mdc-berlin.de
twitter: http://twitter.com/preibischs

On Apr 26, 2018, at 7:07 PM, nebenb <[hidden email]<mailto:[hidden email]>> wrote:

I have, and it seems gets overwhelmed when i have more than 4 images for it
to put together. the worms are all oriented differently so I unfortunately
cannot set a grid for the images to be stitched on. Thanks for the
suggestion though!



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